发明名称 |
SYSTEM FOR TRANSPOSING HYPERACTIVE RECOMBINANT DERIVATIVES OF MOS-1 TRANSPOSON |
摘要 |
The invention concerns a system for transposing a hyperactive recombinant derivative of Mos-1 transposon, comprising at least the two following partners: a) a Mos-1 pseudo-transposon in which an exogenous nucleotide sequence of interest replaces the nucleotide sequence encoding the original Mos-1 transposase; and b) a Mos-1 tranposase provided in trans in said pseudo-transposon, at least one of said partners being appropriately genetically modified to improve the transposition frequency of said exogenous nucleotide sequence of interest. Additionally to such systems, the invention concerns hyperactive Mos-1 transposons, hyperactive Mos-1 transposases, kits. The invention further concerns the use of one or more of abovementioned means for carrying out sequence transpositions, and more particularly efficient gene transfers.
|
申请公布号 |
US2010291682(A1) |
申请公布日期 |
2010.11.18 |
申请号 |
US20070227271 |
申请日期 |
2007.05.15 |
申请人 |
BIGOT SYLVIE MARIE-LOUISE;BIGOT YVES PHILIPPE MARCEL;BONNIN-ROULEUX FLORENCE;GERMON STEPHANIE ODILE REBECCA;JERGOT GWENHAEL |
发明人 |
BIGOT SYLVIE MARIE-LOUISE;BIGOT YVES PHILIPPE MARCEL;BONNIN-ROULEUX FLORENCE;GERMON STEPHANIE ODILE REBECCA;JERGOT GWENHAEL |
分类号 |
C07H21/04;C12N1/21;C12N5/10;C12N9/12;C12N15/63;C12N15/79 |
主分类号 |
C07H21/04 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|