发明名称 |
Method of amidated peptide biosynthesis and delivery in vivo: endomorphin-2 for pain therapy |
摘要 |
The invention provides an expression cassette comprising a DNA sequence encoding amino acids 1-99 of human preproenkephalin, a DNA sequence encoding a precursor of a carboxy-amidated peptide flanked by dibasic cleavage sites and optionally a DNA sequence encoding a marker protein (such as Enhanced Green Fluorescent Protein (GFP)) all in operable linkage and under control of a promoter. Where the encoded precursor of a carboxy-amidated peptide is an agonist for an opioid receptor, the invention further provides a method of treating neuropathic pain by administering the gene transfer vector comprising such an expression cassette to a patient. The invention also provides a method for detecting a peptide having a desired effect comprising introducing a library of DNA sequences encoding one or more precursors of carboxy-amidated peptides into host cells; expressing the carboxy-amidated peptides encoded in the library to provide expression products; and screening from the polypeptide expression products for the desired effect.
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申请公布号 |
US7825231(B2) |
申请公布日期 |
2010.11.02 |
申请号 |
US20060445837 |
申请日期 |
2006.06.01 |
申请人 |
REGENTS OF THE UNIVERSITY OF MICHIGAN |
发明人 |
WOLFE DARREN P.;GLORIOSO JOSEPH C.;FINK DAVID J. |
分类号 |
C07H21/04;A61K31/711;C12N15/12;C12N15/62 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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