| 摘要 |
Problems to be Solved There is provided a method for separating the complex containing the analyte (or the analogue) in the blood-derived sample and the labeling substances, etc., and coexisting substances in a blood-derived sample, rapidly, simply and conveniently and in high precision by a isotachophoresis (ITP); and a measuring method for the analyte in said sample in high precision and in high sensitivity, based on amount of the complex separated or amount of the free labeling substance-containing molecules, which were not involved in formation of said complex. Method for Solving the Problem A method for separating a complex which comprises, concentrating the following complex, while separating a complex containing an analyte (or the analogue) in the blood-derived sample, the substances which are capable of forming the complex (CFS) with said analyte and the substances which are capable of changing electrophoretic mobility of the analyte (or the analogue), from the free labeling substance-containing molecules (the labeled CFS, the labeled analogue and the analyte-labeled CFS complex) which were not involved in formation of the complex including the labeling substances and the coexisting substances in said sample, by ITP, in the presence of an 2-(N-morpholino)ethane sulfonate (MES) ion and/or a glutamate ion.
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