发明名称 |
METHODS FOR MITOCHONDRIAL DNA REPLACEMENT IN OOCYTES |
摘要 |
Methods are provided for producing a primate oocyte in vitro. The methods include removing nuclear DNA from a recipient primate oocyte from a first primate in a manner that does not lower levels of maturation promoting factor (MPF) to form an enucleated recipient primate oocyte. The recipient primate oocyte is enucleated using a non-UV-based spindle imaging system. Nuclear genetic material or DNA including chromosomes from a donor primate oocyte arrested at metaphase II from a second primate is isolated in the form of the karyoplast and introduced into the enucleated recipient primate oocyte. Introduction of the chromosomes is performed using a fusogenic agent or electroporation to produce a hybrid oocyte. |
申请公布号 |
WO2010124123(A1) |
申请公布日期 |
2010.10.28 |
申请号 |
WO2010US32101 |
申请日期 |
2010.04.22 |
申请人 |
OREGON HEALTH & SCIENCE UNIVERSITY;MITALIPOV, SHOUKHRAT M. |
发明人 |
MITALIPOV, SHOUKHRAT M. |
分类号 |
C12N5/075;C12N15/00 |
主分类号 |
C12N5/075 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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