| 摘要 |
A fluorescence focal modulation microscopy system and method is disclosed for high resolution molecular imaging of thick biological tissues with single photon excited fluorescence. Optical sectioning and diffraction limited spatial resolution are retained for imaging inside a multiple-scattering medium by the use of focal modulation, a technique for suppressing the background fluorescence signal excited by the scattered light. The focal modulation microscopy system has a spatial phase modulator inserted in the excitation light path, which varies the spatial distribution of coherent excitation light around the focal volume periodically at a preset frequency. A fluorescence focal modulation image is formed on a display with the demodulated fluorescence, while a confocal image is available simultaneously. |
