| 摘要 |
Disclosed are processes for generating and detecting recombinant DNA sequences in Saccharomyces cerevisiae comprising the steps of: a) generating first diploid S. cerevisiae cells bearing in a defined locus of their genome a first recombination cassette comprising a first DNA sequence to be recombined, which is flanked by at least a first and a second marker sequences, and in an allelic position a second recombination cassette comprising a second DNA sequence to be recombined, which is flanked by at least a third and a fourth marker sequences, b) inducing the sporulation of the first diploid cells obtained in a), and c) isolating haploid cells containing recombination cassettes in which first recombined DNA sequences are flanked by at least the first and fourth marker sequences, and haploid cells containing recombination cassettes in which second recombined DNA sequences are flanked by at least the second and the third marker sequences.
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