| 摘要 |
The invention relates to a method for the purification and/or the concentration of protein inclusion bodies in a highly concentrated, particle-containing solution. The suspension containing the protein inclusion bodies is tangentially passed over one or a plurality of semi-permeable membranes, such that the protein inclusion body is retained behind the membranes and substances with a lower molecular weight pass through the membrane and/or are adsorbed on the membrane, resulting in a purified and/or concentrated protein inclusion body suspension. In a similar manner cell wall particles may be separated from a protein inclusion body suspension. The invention further relates to the use of a crossflow microfiltration unit for the purification and/or concentration of protein inclusion bodies in a suspension and the separation of cell wall particles from a protein inclusion body preparation.
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