A microfluidic cell sorter has a microfluidic structure (500) with a sample input channel (1 1 0) leading into an observation region (214), two buffer channels (1 1 8) configured to hydrodynamically focus a sample target cell (208) within the observation region, and at least two output channels (1 1 4). Apparatus directs the target cell into a selected output channel based on a cell sorting control signal (61 6). A CARS pulse source (524) generates CARS pulses (526), which are directed to the target cell within the observation region. A detector (530) detects CARS illumination scattered from the target signal and generates a spectrum signal based on the detected illumination. A processor (61 4) identifies the target cell based on the spectrum signal and generates the cell sorting control signal based on the identity of the target cell.
申请公布号
WO2010053796(A3)
申请公布日期
2010.09.02
申请号
WO2009US62419
申请日期
2009.10.28
申请人
THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODYCORPORATE;LEI, TIM, CHIFONG;GIBSON, EMILY, ABBOTT