NUCLEIC ACID BINDING PROTEINS

摘要

The invention provides a method for preparing a nucleic acid binding protein of the Cys2-His2 zinc finger class capable of binding to a nucleic acid quadruplet in a target nucleic acid sequence, wherein binding to base 4 of the quadruplet by an .alpha.-helical zinc finger nucleic acid binding motif in the protein is determined as follows: if base 4 in the quadruplet is A, then position +6 in the .alpha.-helix is Gln and position ++2 is not Asp; and if base 4 in the quadruplet is C, then position +6 in the .alpha.-helix may be any residue, as long as position ++2 in the .alpha.-helix is not Asp.