NOVEL NUCLEIC ACIDS FOR ENHANCING GENE EXPRESSIONS AND A METHOD FOR PROTEIN PRODUCTION USING THE SAME

摘要

PURPOSE: A novel stab nucleic acid molecule enhancing gene expression is provided to enhance expression level of downstream target gene and RNA stabilization. CONSTITUTION: A novel stab nucleic acid molecule having STAB-SD(Shine Dalgarno) has a base sequence of sequence number 1. The novel stab nucleic acid molecule having STAB-SD activity comprises a stab nucleic acid molecule and 5' nucleic acid molecule of cry3Ba structure gene connected to 3' of nucleic acid molecule. A method for producing the target protein comprises: a step of preparing a vector having stab nucleic acid molecule; a step of introducing the vector to a host cell; a step of collecting the target protein encoded by target gene; and a step of purifying the target protein using a column.