DNA DIVERSITY DECTECTION METHOD OF AZUKI-BEAN AND IDENTIFICATION METHOD OF AZUKI-BEAN

摘要

PURPOSE: A method for detecting DNA polymorphism by real time PCR using SSR primer pair is provided to effectively detect DNA amplification polymorphism and to use in determining the species of Vigna angularis (Willd.) Ohwi and Ohashi. CONSTITUTION: A method for detecting DNA polymorphism comprises: a step of isolating genome DNA from Vigna angularis (Willd.) Ohwi and Ohashi; a step of performing real time PCR using SSR primer pair; and a step of separating PCR product. An SSR primer pair is a primer pair of sequence number 1 and 2, sequence numbers 3 and 4, or sequence numbers 5 and 6. The primer pair is derived from Vigna angularis (Willd.) Ohwi and Ohashi.