DETECTION AND/OR QUANTIFICATION METHOD OF TARGET MOLECULES ON A SOLID SUPPORT

摘要

The invention relates a method for detecting and/or quantifying different target molecules being bound at different locations (spots) of a surface of an optically transparent solid support having a refractive index n1 by specific interaction with capture molecules being immobilized on the solid support surface, said solid support being in contact with a solution having refractive index n2, whereby n1 > n2 and wherein the target molecules are in contact with the capture molecules, said method comprising the steps of: a) illuminating homogeneously the surface of the support on which the target molecules are bound, thereby causing homogeneous excitation of the target molecule; b) detecting light emitted from the target molecules in at least four different locations (spots) in the following way: - collecting the emitted light through a side of said support which is inclined relative to the surface of the support on which the target molecules are bound, - focusing the emitted light by a lens on a detector surface which is positioned at an observation angle ?obin relative to the normal to the said solid support surface in the support, such that 90° > ?obin > sin-1 (n2/n1), - obtaining an image of at least 4 different locations (spots) being spatially discriminated onto the surface of the detector.