RECOMBINANT VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) SECRETING Escherichia coli BL21 (pVEGF-A165) CELL STRAIN

摘要

FIELD: chemistry; biochemistry. ^ SUBSTANCE: invention pertains to biotechnology. In particular, the invention relates to an Escherichia coli BL21 (pVEGF-A165) strain and can be used to produce a vascular endothelial growth factor - GST-VEGF-A165 protein. A novel Escherichia coli BL21 (pVEGF-A165) cell strain is obtained, which is transformed by the pGEX-VEGF-A165 plasmid. This strain produces a recombinant GST-VEGF-A165 protein. ^ EFFECT: invention enables to obtain a Escherichia coli BL21 (pVEGF-A165) strain which is stably transformed by plasmid which codes VEGF, and which secrete this factor in extracellular space when cultured in vitro. ^ 3 dwg, 4 ex