METHOD OF EXPRESSING PROTEINS WITH DISULFIDE BRIDGES WITH ENHANCED YIELDS AND ACTIVITY
摘要
Provided herein are methods for expressing proteins with disulfide bridges such as Vicrostatin (VCN), a chimeric variant of native snake venom disintegrin Contortrostatin (CN). The methods include what is believed to be a more efficient natural selection process that results in generating increased amounts of correctly-folded active conformers of proteins with disulfide bridges. In an aspect, this is achieved by growing Origami B cells in a more optimal redox environment during the induction of heterologous recombinant protein production.
申请公布号
WO2010056901(A3)
申请公布日期
2010.07.22
申请号
WO2009US64256
申请日期
2009.11.12
申请人
UNIVERSITY OF SOUTHERN CALIFORNIA;MINEA, RADU O.;SWENSON, STEPHEN D.;MARKLAND, FRANCIS S.
发明人
MINEA, RADU O.;SWENSON, STEPHEN D.;MARKLAND, FRANCIS S.