发明名称 |
METHOD FOR CULTURE OF ANIMAL HEPATOCYTE |
摘要 |
<p>Disclosed is a technique for culturing a hepatocyte three-dimensionally to form a spheroid readily. Also disclosed is a technique for forming a spheroid in which a transporter MRP2 (which is a transporter involved in the bile duct excretion) can be highly expressed compared with conventional techniques. The conditions under which a hepatocyte can be formed into a spheroid on a nanopillar (NP) sheet readily, specifically the conditions relating to the temperature of type-I collagen to be applied on the NP sheet, are now found. In addition, the conditions under which the expression level of a gene involved in the excretion of the formed spheroid can be increased are also found. Specifically, a spheroid is formed in advance, and a biological matrix is subsequently overlaid on the spheroid.</p> |
申请公布号 |
WO2010079602(A1) |
申请公布日期 |
2010.07.15 |
申请号 |
WO2009JP50140 |
申请日期 |
2009.01.08 |
申请人 |
HITACHI, LTD.;TAKAHASHI, RYOSUKE;HISADA, AKIKO;SONODA, HIROSHI |
发明人 |
TAKAHASHI, RYOSUKE;HISADA, AKIKO;SONODA, HIROSHI |
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