| 摘要 |
The present invention relates to a method and an apparatus for processing and metrically quantifying images of objects containing clusters of points/spots, such as biological specimens comprising cluster of cells, in particular of human or animal origin, or images thereof. In particular, the present invention relates to a method for processing images of irregularly shaped objects in the form of at least one cluster of punctiform or spot-shaped objects, comprising a stage of acquisition of a digital image of said objects, a stage of image elaboration (IMA-EL) for quantizing said digital image to 1 bit and a stage of metrical processing of said 1-bit quantized image, wherein said stage of metrical processing comprises a stage of object's metrical quantification (QUANT) that on its turn comprises: -a stage of triangularization (TRIANG) for transforming the said at least one cluster of punctiform or spot-shaped objects into a grid of triangles wherein the apexes of the triangles correspond to the center of said punctiform or spot-shaped objects; -a stage of parameter calculation (PAR-CLC) for calculating at least one of the following parameters: -external perimeter of the said grid of triangles; -area (AC) of the said grid of triangles; -area (ACINF) of the said punctiform or spot-shaped objects inside the said grid of triangles; -area (APINF) of the isolated punctiform or spot-shaped objects outside the said at least one cluster; -density (DC) of the said punctiform or spot-shaped objects inside the said at least one cluster. |
