发明名称 |
METHODS FOR CONTROL OF FLUX IN METABOLIC PATHWAYS |
摘要 |
The invention pertains to a method for preparing cells that can be used as biocatalysts by inducing in them a growth-decoupled state, in which interferase inhibits the expression of genes except the ones that code for the pathway enzymes of interest. mRNAs that code for interferase-resistant products are overexpressed in the background of a metabolically-frozen cell. Enzymes that compete for a substrate or product of the pathway of interest may be altered such that the enzyme is sensitive to a site-specific protease, which protease is inducible in the host cell. |
申请公布号 |
WO2010077806(A1) |
申请公布日期 |
2010.07.08 |
申请号 |
WO2009US67841 |
申请日期 |
2009.12.14 |
申请人 |
GREENLIGHT BIOSCIENCES, INC.;KLEIN-MARCUSCHAMER, DANIEL |
发明人 |
KLEIN-MARCUSCHAMER, DANIEL |
分类号 |
C12N1/00;C07H21/00;C12N9/22;C12P19/34 |
主分类号 |
C12N1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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