A PREPARATION METHOD OF SOLUBILITY RECOMBINANT PROTEIN BY USE OF ASPARTATE CARBAMOYLTRANSFERASE CATALYTIC CHAIN AS A FUSION EXPRESSION PARTNER

摘要

PURPOSE: A method for preparing a recombinant protein using aspartate carbamoyltransferase catalytic chain(PyrB) as a fusion expression partner is provided to improve solubility and folding ability. CONSTITUTION: A method for preparing a recombinant protein comprises: a step of preparing an expression vector having aspartate carbamoyltransferase catalytic chain(PyrB) gene and gene of foreign protein; a step of introducing the expression vector to a host cell; a step of culturing the transformant to induce the recombinant protein. The PyrB is denoted by sequence number 1. The foreign protein is granulocyte colony-stimulating factor(hGCSF). The expression vector additionally contains a protein restriction enzyme recognizing site between the PyB gene and foreign protein gene. The host cell is E. coli.