| 摘要 |
The present invention provides genetically modified yeast cells, and methods of using those yeast cells, to produce 1-butanol. The yeast cell can be selected from the genera Saccharomyces, Candida, Pichia, Kluyveromyces, Issatchenkia, Yarrowia, Rhodotorula, Hansenula, Schizochytrium, or Thraustochytrium. The yeast cell of the invention overexpresses at least one enzyme that catalyzes one or more butanoate pathways selected from the group consisting of pyruvate to acetyl-CoA, acetyl-CoA to acetoacetyl-CoA, acetoacetyl-CoA to 3-hydroxybutanoyl-CoA, 3-hydroxybutanoyl-CoA to crotonoyl-CoA, crotonoyl-CoA to butyryl-CoA to butyraldehyde, and butyraldehyde to 1-butanol. Some embodiments overexpress enzymes that are endogenous to the bacterium Clostridium acetobutylicum. The genetically modified yeast cell can further be subjected to other desired genetic changes, such as deletion or disruption of one or more native PDC genes.
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