发明名称 |
PROCESS FOR THE ENZYMATIC PRODUCTION OF CYCLIC DIGUANOSINE MONOPHOSPHATE EMPLOYING A DIGUANYLATE CYCLASE COMPRISING A MUTATED RXXD MOTIF |
摘要 |
A process is disclosed for the production of cyclic di-guanosine monophosphate (c-di-GMP) without the use of protecting groups by means of an enzymatic synthesis. The process comprises the coupling of two guanosine triphosphate (GTP) molecules so as to form a c-di-GMP molecule. This is done under the influence of a mutant diguanylate cyclase (DGC) comprising the amino acid sequence V153M154G155G156. It has been found that the DGC is obtainable from inclusion bodies, and therewith can be made available in amounts sufficient to improve the c-di-GMP synthesis. Particularly, the latter synthesis can be conducted in a one-pot method starting from commercially available bulk chemicals and allows upscaling to a commercial production scale. |
申请公布号 |
WO2010066666(A1) |
申请公布日期 |
2010.06.17 |
申请号 |
WO2009EP66492 |
申请日期 |
2009.12.07 |
申请人 |
INTERVET INTERNATIONAL B.V.;ILG, THOMAS SIMON;SPEHR, VOLKER;WARRASS, RALF |
发明人 |
ILG, THOMAS SIMON;SPEHR, VOLKER;WARRASS, RALF |
分类号 |
C12N15/54;C07H19/207;C12N9/12;C12P19/36 |
主分类号 |
C12N15/54 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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