| 摘要 |
A shock freezer for rapidly freezing a sample preparatory to analysis, comprises an insulated closed container containing cryogenic fluid; a tubing disposed through the container having one end in contact with the cryogenic fluid and another end communicating outside the container, the another end for receiving a needle containing a sample to be shock frozen. The tubing has an inside diameter larger than an outside diameter of a capillary to be inserted therein to provide a space between an inside surface of the tubing and an outside surface of a capillary to be inserted therein to provide passageway for the cryogenic fluid to flow to the outside. An outlet communicates with an interior of the container. A relief valve is operably associated with the outlet, the relief valve being normally open to relieve pressure within the container, and the relief valve being closed when a capillary to be inserted containing a sample to be frozen is inserted into the tubing through the another end to allow pressure within the container to build up and force the cryogenic fluid to flow upwardly through the tubing through the space, thereby to freeze a sample in a capillary to be inserted.
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