<p>A mixing cartridge (200) for mixing a sample with a first reagent corresponding to a test item of the sample to be determined. The mixing cartridge (200) comprises: a member forming a microchannel (1) for passing through a solution containing either the sample and/or the first reagent; a sample tank (5) and a sample pump (15), which supply the sample to the microchannel (1); a first reagent tank (4) and a first reagent pump (14), which supply the first reagent to the microchannel (1); and a first waste microchannel (6), which communicates with the microchannel (1) and separates a part of the solution from the remaining part thereof passing through the microchannel (1) by capillary action.</p>