| 摘要 |
The present invention provides a method for amplifying a pool of polynucleotide molecules in a sample, characterized by the steps of a) obtaining a sample or RNA and reverse transcription of entire RNA molecules thus creating full length cDNA or obtaining a sample of full length cDNA, b) tailing the 3' end of the transcribed cDNA with a polynucleotide tail after the 3' end, c) amplification of the cDNA using a pair of primers, wherein a first 3' primer is specific for the 5' end of the cDNA and a second 5' primer is specific for the a upstream portion of the polynucleotide tail and the next 1 to 10 nucleotides upstream of the 3'polynucleotide tail of the cDNA. |
