METHOD OF PRODUCING SERUM-FREE INSULIN-FREE FACTOR VII

摘要

The invention provides methods and compositions for producing recombinant human Factor VII (FVII) comprising obtaining a Chinese Hamster Ovary (CHO) cell line that expresses recombinant human FVII, culturing said CHO cell in serum-free media that lacks insulin wherein the production of said FVII in said CHO cell is comparable to the production of FVII in the presence of insulin.