| 摘要 |
The present invention relates to gram-negative bacterial mutants resistant to one or more stress conditions, e.g., CO2, acid pH, and high osmolarity. Gram-negative bacterial mutants with reduced TNF-alpha induction having a lipid biosynthesis gene mutation, including, but not limited to msbB, are rendered stress-resistant by a zwf mutation. The stress-resistant gram-negative bacterial mutants are preferably attenuated, and may be used for prophylaxis or treatment of a virally induced disease by administering stress-resistant gram-negative bacterial mutants. Methods for prophylaxis or treatment of a virally induced disease in a subject are provided comprising administering to said subject one or more stress-resistant gram-negative bacterial mutants as vectors for the delivery of one or more therapeutic molecules. The methods of the invention provide more efficient delivery of therapeutic molecules by stress-resistant gram-negative bacterial mutants engineered to express said therapeutic molecules. |
