MODIFIED FLAVIN-ADENINE-DINUCLEOTIDE-DEPENDENT GLUCOSE DEHYDROGENASE

摘要

Disclosed are: a gene for an activated flavin-adenine-dinucleotide-dependent glucose dehydrogenase (GDH) (FADGDH), which is produced by inserting a specific amino acid sequence into a protein derived from Aspergillus oryzae, and an activated FADGDH comprising an amino acid sequence encoded by the gene; and a gene for a modified FADGDH having improved affinity for a substrate and/or an improved specific activity, which is produced by replacing a specific amino acid residue in the activated FADGDH by another amino acid residue, and an activated FADGDH comprising an amino acid sequence encoded by the gene.  It becomes possible to provide a GDH which has excellent affinity for a substrate and an excellent specific activity and is unaffected by dissolved oxygen.