发明名称 |
METHOD FOR ISOLATING AND CULTURING UNCULTURABLE MICROORGANISMS |
摘要 |
The invention provides a method for isolating and culturing a previously unculturable microorganism, which comprises: (i) collecting a sample from an environmental source; (ii) counting/estimating the number of microorganisms in the sample; (iii) diluting the sample in an appropriate medium; (iv) adding a gelating agent such as to entrap one or more microorganisms within a sphere of the gelating agent; (v) coating the spheres containing the entrapped microorganism(s) with a natural or synthetic polymer to form a polymeric membrane; (vi) incubating the coated spheres in the original environment for an appropriate time; (vii) cutting the spheres and scanning for microorganisms colonies; and (viii) isolating the microorganisms, and repeating steps (iii) to (vii) until a pure clone of said previously unculturable microorganism is obtained.
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申请公布号 |
US2010120037(A1) |
申请公布日期 |
2010.05.13 |
申请号 |
US20090537646 |
申请日期 |
2009.08.07 |
申请人 |
KUSHMARO ARIEL;GERESH SHIMONA;GERESH SHAUL |
发明人 |
KUSHMARO ARIEL;GERESH SHIMONA;GERESH SHAUL |
分类号 |
C12Q1/68;C12N1/00;C12N1/20;C12P21/04;C40B40/02 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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