发明名称 |
PROCEDURE FOR LONG TERM CORNEAL CULTURE |
摘要 |
A method for long-term multi-species cornea culture and preservation is provided. Excised mammalian corneas are preserved with structural integrity by filling the endothelial cavity with a plug material. Plugged corneas are sterile-cultured for more than 21 days via an air-interface culture where nutrients from a culture media are supplied to corneal tissue primarily through the plug. Plugged corneas are incubated in culture dishes with a customized media continuously in contact with the ocular sclera and corneal plug, and periodically bathing the corneal surface epithelia. An animal-alternative toxicology assay is also provided using excised porcine corneas capable of assessing ocular injury reversibility within 21 days. Further provided is a method of mammalian corneal preservation for excised human corneas extending their storage life to three to four weeks.
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申请公布号 |
US2010120013(A1) |
申请公布日期 |
2010.05.13 |
申请号 |
US20080266906 |
申请日期 |
2008.11.07 |
申请人 |
MB RESEARCH LABORATORIES, INC. |
发明人 |
CERVEN DANIEL R.;PIEHL MICHELLE A.;GILOTTI ALBERT C.;DONOVAN ALISON L.;DEGEORGE GEORGE L.;CARATHERS MICHEAL R. |
分类号 |
A01N1/02;C12Q1/02 |
主分类号 |
A01N1/02 |
代理机构 |
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地址 |
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