| 摘要 |
A method for the detection and quantification of RNA via micro-arrays, wherein a first DNA molecule is added to a RNA-pool to be tested, which first DNA molecule is complementary to at least a first segment of a RNA of interest, as well as a second DNA molecule complementary to a second segment of the RNA of interest, which second segment is different from said first segment and further has at the 3′ and 5′ ends specific nucleotide sequences, which are not complementary to the RNA of interest. The DNA molecules are contacted with the RNA-pool under conditions allowing hybridization of complementary strands. Afterwards all RNA molecules are removed to which said first DNA molecule has not hybridized. After releasing of the second DNA molecules, they are contacted with an array having at specific locations thereof probes specific for particular nucleotide sequences.
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