| 摘要 |
A mutant thermally stable DNA polymerase having at least one Lysine to Arginine substitution corresponding to at least one of amino acid position 505, 540 or 542 of Thermus aquaticus Taq polymerase A. Said polymerase can also be chemically modified to substantially reduced polymerase activity at ambient temperatures, wherein the chemical modification modifies at least one Lysine residue of the polymerase and said reduced polymerase activity is reversible upon heating at a temperature of at least 50 °C. Methods for using and kits containing the mutant DNA polymerase and chemically modified, mutant DNA polymerase for primer extension and amplification, respectively, are also envisioned. |
