| 摘要 |
A protein (I) comprising an amino acid sequence (AS) of 362 amino acids (S1), given in the specification, or (AS) having a chlorohydrin and hydroxycarboxylic ester asymmetric hydrolase activity, where (AS) is an (AS) in which one or several number of amino acid(s) of (S1) is/are deleted, added or substituted or (AS) is an (AS) having 60 % or more of homology with (S1), is new. A new protein (I) comprises either one of an amino acid sequence (AS) chosen from a sequence (S1) of 362 amino acids, given in the specification, and (AS) having a chlorohydrin and hydroxycarboxylic ester asymmetric hydrolase activity, where (AS) is an (AS) in which one or several number of amino acid(s) of (S1) is/are deleted, added or substituted or (AS) is an (AS) having 60 % or more of homology with (S1). Independent claims are also included for the following: (1) a gene (II) comprising either one of base sequences chosen from a base sequence of a fully defined sequence (S2) of 1990 nucleotides as given in the specification, a base sequence which encodes a protein having a chlorohydrin and hydrocarboxylic ester asymmetric hydrolase activity, where the base sequence is a base sequence in which one or several base(s) of (S2) is/are deleted, added or substituted, a base sequence which encodes a protein having a chlorohydrin and hydroxycarboxylic ester asymmetric hydrolase activity and hybridizes with (S2) under stringent conditions, a base sequence which encodes (S1), a base sequence which encodes an amino acid sequence having a chlorohydrin and hydroxycarboxylic ester asymmetric hydrolase activity, where the amino acid sequence is an amino acid sequence in which one or several number of amino acid(s) of (S1) is/are deleted, added or substituted, and a base sequence which encodes an amino acid sequence having a chlorohydrin and hydroxycarboxylic ester asymmetric hydrolase activity, where the amino acid sequence is an amino acid sequence having 60% or ore of homology with (S1); (2) a signal peptide (III) comprising a sequence (S3) of Met-Arg-Lys-Thr-Met-Gln-Arg-Ser-Leu-Leu-Ser-Ala-Leu-Val-Leu-Val-Ala-Ser-Cys-Cys-His-Gly-Ala-Trp-Ala, for a resting strain reaction using (I); (3) a DNA (IV) encoding (III), comprising a fully defined sequence (S4) of 75 nucleotides as given in the specification; (4) a vector (V) comprising (II); (5) a transformant (VI) comprising (I) or (V); and (6) preparation of (I). |
