发明名称 |
METHOD FOR MAINTAINING FOREIGN GENE IN CELL STABLY |
摘要 |
A method for preparing a protein or peptide encoded by a foreign gene by expressing a foreign gene, which comprises the steps of: preparing a recombinant vector comprising in an expressible state a gene encoding an aminoacyl-tRNA synthetase, in which a desired foreign gene has been inserted in an expressible state; preparing a mutant host cell or the like in which a chromosomal gene encoding an aminoacyl-tRNA synthetase has been knocked out; transforming the mutant host cell with the recombinant vector to obtain a transformant; and culturing the transformant to prepare the protein or peptide encoded by the foreign gene. It becomes possible to provide a novel means for permitting the retention of a recombinant DNA cloning vector without employing an antibiotic and without limiting the composition of the medium; and a method for preparing a protein or peptide encoded by a foreign gene by using the means.
|
申请公布号 |
EP2177610(A1) |
申请公布日期 |
2010.04.21 |
申请号 |
EP20080778000 |
申请日期 |
2008.07.09 |
申请人 |
JAPAN AGENCY FOR MARINE-EARTH SCIENCE AND TECHNOLOGY |
发明人 |
HATADA, YUJI;OHTA, YUKARI;HIDAKA, YUKO;NAKAMURA, NOBUYUKI |
分类号 |
C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/00;C12N5/10;C12N9/42 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|