Preparation containing nanoscale particles with electrostatically adhered enzyme

摘要

Improved catalytic enzyme preparations are provided which effectively catalyze reactions carried out in organic solvents. The preparations comprise nanoscale solid support particles (e.g., fumed silica or alumina) having enzyme molecules electrostatically adhered to the surfaces of the particles. The preparations are prepared by mixing together solid support particles and enzyme molecules in an aqueous system under pH conditions establishing opposite charges on the support particles and enzyme molecules, respectively. The water is then removed from the system, preferably by pre-freezing followed by lyophilization to yield free-flowing, particulate catalyst particles which can be used in liquid organic reactions or in packed bed reactors.