| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a thermostable duplex-specific nuclease (DSN) and a method for digesting a nucleic acid using the DSN. <P>SOLUTION: There are provided a protein having a Brachyura-derived duplex-specific nuclease activity, a gene encoding the protein, a recombinant vector comprising the gene, a transformed cell or transduced cell comprising the vector, a method for producing the protein having the duplex-specific nuclease activity, which comprises culturing the transformed cell or transduced cell using a medium and collecting the protein having the duplex-specific nuclease activity from the cultured mixture, a method for digesting a nucleic acid using the protein having duplex-specific nuclease activity, a method for detecting RNA using the DSN, and a reagent kit to be used in these methods. <P>COPYRIGHT: (C)2010,JPO&INPIT |
