发明名称 |
GENOMIC DNA FRAGMENTS CONTAINING REGULATORY AND CODING SEQUENCES FOR THE .BETA.2-SUBUNIT OF THE NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR AND TRANSGENIC ANIMALS MADE USING THESE FRAGMENTS OR MUTATED FRAGMENTS |
摘要 |
Several genes encoding subunits of the neuronal nicotinic acetylcholine receptors have been cloned and regulatory elements involved in the transcription of the .alpha.:2 and .alpha.:7-subunit genes have been described. Yet, the detailed mechanisms governing the neuron-specific transcription and the spatio-temporal expression pattern of these genes remain largely uninvestigated. The .beta.2-subunit is the most widely expressed neuronal nicotinic receptors subunit in the nervous system. We have studied the structural and regulatory properties of the 5' sequence of this gene. A fragment of 1163 bp of upstream sequence is sufficient to drive the cell-specific transcription of a reporter gene in both transient transfection assays and in transgenic mice. Deletion analysis and site-directed mutagenesis of this promoter reveal two negative and one positive element. The positively acting sequence includes one functional E-box. One of the repressor elements is located in the transcribed region and is the NRSE/RE1 sequence already described in promoters of neuronal genes.
|
申请公布号 |
CA2165098(C) |
申请公布日期 |
2010.04.13 |
申请号 |
CA19952165098 |
申请日期 |
1995.12.13 |
申请人 |
INSTITUT PASTEUR |
发明人 |
CHANGEUX, JEAN-PIERRE;PICCIOTTO, MARINA;BESSIS, ALAIN |
分类号 |
A01K67/027;C12N15/12;C07H21/00;C07H21/04;C07K14/705;C07K14/715;C12N5/02;C12N5/10;C12N15/09;C12N15/11;C12N15/85;C12Q1/68 |
主分类号 |
A01K67/027 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|