New compound comprising a duplicable nucleic acid (amplified fragment), where compound comprises a means of locking of polymerization (loop linkage), useful e.g. in complex for diagnosing the amplification of the nucleic acids

摘要

Compound comprising at least one duplicable nucleic acid, named amplified fragment, where the amplified fragment is mainly composed of nucleotides allowing a polymerization of complementary single stranded nucleic acid, the compound comprises at least one means of locking of the polymerization, named loop linkage, mainly constituted substances not allowing polymerization, the amplified fragment comprises a 3' part, a central part, and a 5' part, the parts of 3' and 5' of the amplified fragment all are linked between each other by at least a locking means of polymerization, is new. Independent claims are included for: (1) a complex comprising the compound, and a nucleic acid forming trigger, where the trigger comprises at least one region paired on at least a part of the amplified fragment, the trigger further comprises one unpaired region in amplified fragment for allowing a nucleic acid at least partially complementary of the trigger to be paired at least in a part of the region for releasing the part of the amplified fragment paired with the trigger, preferably by competitive hybridization or duplication of the trigger; (2) a precursor, forming particularly precursor of the compound, where the precursor comprises at least one first and second nucleic acid, end 3' of the first nucleic acid is linked to end 5' of the second nucleic acid by a linkage of a loop linkage; (3) a composition comprising the compound, the complex and/or the precursor in a solvent, preferably aqueous solvent; (4) a diagnostic method comprising implementation of an amplification of nucleic acids comprising the compound, the complex and/or the precursor; (5) a method for detecting the presence of a substance or its mixture, comprising implementing an amplification of nucleic acids contained in the composition comprising the compound, the complex and the precursor; (6) a device carrying out the logical operations or calculation comprising implementing logical operations or calculation of the compound, the complex and/or the precursor; and (7) a logical operator comprising OR, AND, NON AND, comprising implementing the compound, the complex and/or the precursor.