COMPOSITION FOR DETECTION OF M. TUBERCULOSIS COMPLEX OR MYCOBACTERIA GENUS AND SIMULTANEOUS DETECTION METHOD FOR M. TUBERCULOSIS COMPLEX AND MYCOBACTERIA GENUS WITH MULTIPLEX REAL TIME PCR USING THE SAME

摘要

Provided is a composition for detection of M. tuberculosis complex or Mycobacterium genus, comprising (i) a primer and/or probe targeting IS6110 that is an M tuberculosis complex-specific gene, (?) a primer and/or probe targeting rpoB that is a Mycobacterium genus-specific gene, and optionally (i?) a primer and/or probe targeting a plant-derived gene as an internal control. When multiplex real-time PCR is carried out using the composition of the present invention, it is possible to detect M tuberculosis complex as well as Mycobacterium genus by a single roundPCR assay. Therefore, the present invention enables rapid and easy clinical diagnosis with high reliability.