METHOD FOR PRODUCTION OF L-LYSINE USING METHANOL-UTILIZING BACTERIUM

摘要

Disclosed is a method for production of L-lysine, comprising the steps of cultivating a methanol-utilizing bacterium in a culture medium to produce and accumulate L-lysine in the culture medium and collecting the L-lysine from the culture medium, wherein the methanol-utilizing bacterium carries both of DNA encoding a dihydrodipicolinate synthetase which is desensitized to feedback inhibition by L-lysine and DNA encoding a mutant LysE protein that can enhance the excretion of L-lysine out of a cell of a methanol-utilizing bacterium when introduced into the methanol-utilizing bacterium and is modified so as to increase the intracellular activities of diaminopimelic acid dehydrogenase, diaminopimelic acid decarboxylase, dihydrodipicolinic acid reductase and aspartate-semialdehyde dehydrogenase.