ADCC with modified NK92 cells

摘要

The present invention relates to a method for producing Fc³IIIa receptor expressing NK92 cell comprising a) providing a plasmid which comprises DNA encoding Fc³IIIa receptor and a selection marker and b) introducing said plasmid with electroporation into NK92 cells Furthermore, the invention relates to an in vitro method for assessing the efficacy of an antibody to induce NK mediated ADCC comprising: a) producing an effector cell with the described producing method for modified NK92 cells, wherein said effector cell is a modified NK92 cell expressing Fc³IIIa receptors, b)exposing a labeled target cell to an antibody of interest and the effector cell, c) monitoring the target cell for cytotoxicity, cytolysis or apoptosis with a nonradioactive readout-system.