MUTANT TRANSGLUTAMINASE FROM STREPTOVERTICILLIUM HAVING MODIFIED SUBSTRATE SPECIFICITY

摘要

The present invention relates to a method for designing and preparing mutan t transglutaminases on the basis of the three-dimensional structure of MTG derived from Streptoverticillium mobaraense (MTG), and the mutant MTG thus prepared. The present invention provides a method for modifying MTG on the basis of th e three-dimensional structure, and transglutaminase having reactivity on the substrate improved by the method. In the present invention, the binding site of MTG fo r the substrate is extrapolated based on the three-dimensional structure obtained by X-ray crystal structure analysis of MTG crystals, and the mutant transglutaminases are designed and produced by replacing, inserting or deleting amino acid residue s positioned at the substrate-binding site of the transglutaminase.