| 摘要 |
The present invention refers to an immuno-chromatographic detection cup and its use for simultaneously detecting at least one antigen, e.g. tuberculosis antigen, malaria antigen and pneumonia antigen, and at least one antibody, e.g. HIV antibody, HCV antibody and H. pylori antibody, in a urine sample. The urinary detection cup comprises (a) a sample-collecting container; (b) a conjugate releasing pad comprising a gold labelled antibody X and an oligo-nucleotide linked antibody X′, wherein both antibodies are directed against the same antigen, and a gold labelled antigen Y and an oligo-nucleotide linked antigen Y′, wherein both antigens are recognized by the same antibody; (c) a test means inside the container separated from the conjugate releasing pad, which test means comprises a region comprising an oligo-nucleotide complementary to the oligo-nucleotide linked to antibody X′, a region comprising an oligo-nucleotide complementary to the oligo-nucleotide linked antigen Y′, and at least one region comprising a control antibody and/or a control antigen; and (d) at least two sample absorbent pads linked to the test device at different positions.
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