MODIFIED BIOTIN-BINDING PROTEIN

摘要

Disclosed is a modified biotin-binding protein. The modified biotin-binding protein is characterized by comprising the amino acid sequence depicted in SEQ ID NO:2, an amino acid sequence produced by introducing the mutation of one to several amino acid residues into the aforementioned sequence, or an amino acid sequence having a 80% or more identity with the aforementioned sequence and having a biotin-binding activity, wherein at least one residue selected from the group consisting of the residues 1) to 4) shown below is substituted by an acidic amino acid residue or a neutral amino acid residue: 1) an arginine residue located at position-104 in SEQ ID NO:2; 2) a lysine residue located at position-141 in SEQ ID NO:2; 3) a lysine residue located at position-26 in SEQ ID NO:2; and 4) a lysine residue located at position-73 in SEQ ID NO:2.