发明名称 |
Pulsing of Bile Compartments in Sandwich-Cultured Hepatocytes |
摘要 |
A method of pulsing cultured hepatocytes, such as sandwich-cultured hepatocytes. The method includes providing a culture of hepatocytes, the culture having at least one bile canaliculus; exposing the culture of hepatocytes to a calcium-free buffer, whereby the contents of the at least one bile canaliculus are released; and removing the calcium-free buffer Pulsing cultured hepatocytes can reduce cholestasis arid can provide an in vitro culture of hepatocytes the more closely reflects in vivo hepatocyte characteristics.
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申请公布号 |
US2010035293(A1) |
申请公布日期 |
2010.02.11 |
申请号 |
US20070308138 |
申请日期 |
2007.06.08 |
申请人 |
BROUWER KIM;TIAN XIANBIN;ZHANG PELJIN;HOFFMASTER KEITH |
发明人 |
BROUWER KIM;TIAN XIANBIN;ZHANG PELJIN;HOFFMASTER KEITH |
分类号 |
C12Q1/02;C12N5/07;C12N5/071 |
主分类号 |
C12Q1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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