THE METHOD FOR PREPARING PATTERNED PROTEIN NANOARRAYS WITH ENHANCED PROTEIN ADSORPTION ON A SELF-ASSEMBLED BLOCK COPOLYMER THIN FILM AND THE PATTERNED PROTEIN NANOARRAYS THEREBY

摘要

PURPOSE: A method for preparing protein nanoarray which protein adsorption is improved on self-assembly block copolymer thin film is provided to selectively adsorb protein on the copolymer. CONSTITUTION: A method for preparing protein nanoarray having improved protein adsorption comprises: a step 1 of spin-coating random copolymer which is dissolved in solvent to form neutral surface layer; a step 2 of control molecular weight(Mn) of block copolymer to improve protein adsorption; a step 3 of performing thermal treatment of block copolymer to induce self-assembly and preparing a template which linterf. is controlled at 8-84 μm/μm^2; a step 4 of dipping the template in a protein solution to prepare protein nano array in which a protein is adsorbed; and a step 5 of washing protein nano array to form nano pattern. The random copolymer is a polystyrene-polybenzocyclobutene-polymethylmethacrylate(P(S-r-BCB-r-MMA)) or polystyrene-block-polymethylmethacrylate random copolymer. The block copolymer is polystyrene-block-polymethylmethacrylate.