发明名称 |
Stabil genomisk integrering af multipie polynukleotidkopier |
摘要 |
Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; means for carrying out the methods, resulting cells, and methods for producing a polypeptide of interest using the resulting cells. |
申请公布号 |
DK1805296(T3) |
申请公布日期 |
2010.01.18 |
申请号 |
DK20050794647T |
申请日期 |
2005.10.19 |
申请人 |
NOVOZYMES A/S |
发明人 |
BREUENER, ANNE;RASMUSSEN, MICHAEL, DOLBERG |
分类号 |
C12N1/21;C12N15/75;C12N15/90;C12R1/10;C12R1/125 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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