| 摘要 |
The present invention relates to genetic modification for industrial applications of moderately thermophilic Bacillus species that are facultative anaerobic and homolactic. The present invention comprises a method for modifying moderately thermophilic Bacillus species that are facultative anaerobic and homolactic by genetic engineering comprising: introducing a DNA cloned in a thermosensitive plasmid system containing a pSH71 replicon or a homologue thereof into cells of a moderately thermophilic Bacillus species that is facultative anaerobic and homolactic; culturing the cells on a selective medium at a permissive temperature for plasmid replication to select transformed cells capable of growing on said selective medium at said permissive temperature; culturing said transformed cells on a selective medium at a non-permissive temperature for plasmid replication to select transformed cells capable of growing on said selective medium at said non-permissive temperature. The method according to the invention can be used to modify the Bacilli for R-lactic acid production, production of other organic acids than lactic acid, alcohol, enzymes, amino acids, and vitamins. In particular, the invention provides a method wherein the Bacillus species is modified by replacing the gene encoding S-lactate dehydrogenase by a DNA construct comprising a DNA sequence encoding R-lactate dehydrot genase. |
