METHODS AND COMPOSITIONS FOR ADENO-ASSOCIATED VIRUS (AAV) WITH HI LOOP MUTATIONS

摘要

The invention provides modified AAV capsid proteins comprising substitutions in the HI loop. Suitable substitutions include affinity tags, sequences that facilitate detection and/or targeting peptides. The invention also provides virus capsids and virus vectors comprising the modified AAV capsid proteins and methods of using the same. Further provided are methods of purifying the modified AAV capsid subunits, virus capsids and virus vectors of the invention.