PROCESS FOR PRODUCTION OF LIPSTATIN AND MICROORGANISMS THEREFORE

摘要

This invention relates to improved processes for the production of lipstatin and/or orlistat and means therefore. In particular, the invention relates to microorganisms characterized by abolished or reduced activity of the BKD complex, e.g. mutated branched- chain 2-oxo acid dehydrogenase (BKD) having reduced or abolished activity, nucleotide sequences suitable for achieving reduced or abolished BKD activity, and microorganisms having reduced or abolished BKD activity. The invention in particular relates to lipstatin- producing microorganisms of the genus Streptomyces. Furthermore, this invention relates to the use of these nucleotide sequences, microorganisms or BKD having reduced or abolished activity in the production of lipstatin and/or orlistat.