Improved method for the prevention of carryover contamination in nucleic acid amplification technologies

摘要

An improved method of preventing carryover contamination of an amplification reaction involves treating uracil-containing DNA with uracil-N-DNA glycosylase and heating the DNA in the presence of polyamines, such as spermidine, spermine and the like. Alternatively, the after treatment with uracil-N-DNA glycosylase, the reaction is further incubated with an enzyme having AP lyase activity.