Nucleic acid sequencing using chemically-sensitive field effect transistors (chemFETs)

摘要

A method for sequencing a nucleic acid using chemFET (e.g. ISFET, pHFET) arrays based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates. Also claimed is an apparatus for detection of ion pulses comprising a laminar fluid flow system; a method for manufacturing a sequencing device comprising generating wells in a glass material on top of an array of transistors; a method for manufacturing an array of FETs coupled to a floating gate; and reaction methods for determining nucleotide sequences.